Over the course of the semester you will learn fundamentals such as cloning and DNA sequencing. With your lab partners, you will design and conduct your own experiment to study the regulation of heat shock protein 70 (HSP70) in a mammalian cell line. You will determine how your experimental treatments regulate HSP70 mRNA and protein levels using quantitative RT-PCR and western immunoblotting, respectively. In the last laboratory session of the semester, each lab group will present their results to their classmates using a PowerPoint presentation.
Here, we investigated the impact of lignin reduction on mechanical properties of poplar downregulated for CINNAMYL ALCOHOL DEHYDROGENASE . A comprehensive analysis of mechanical, structural, and chemical properties revealed that lignin alteration affects tensile properties, but that this effect can only be observed for cell walls with sufficiently high cellulose microfibril angle. The study deepens our understanding of the mechanical interaction of cell wall polymers and of potential challenges in unraveling the impact of cell wall engineering.